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ab5370 goat polyclonal anti il 27 r d systems  (R&D Systems)


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    R&D Systems ab5370 goat polyclonal anti il 27 r d systems
    Ab5370 Goat Polyclonal Anti Il 27 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+27+goat+anti+human/Human+IL-27+Antibody/pm35007556-271-29-33
    Average 94 stars, based on 25 article reviews
    ab5370 goat polyclonal anti il 27 r d systems - by Bioz Stars, 2026-10
    94/100 stars

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    Purification:

    Article Title: T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer
    Article Snippet: All statistical analysis was performed using Graphpad Prism software. .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ultra-LEAFTM Purified anti- human TNF-α Antibody Biolegend Biolegend Cat#502815 Clone MAb1 RRID:AB_2814397 (BioLegend Cat. No. 502805) Ultra-LEAFTM Purified antihuman IFN-γ Antibody Biolegend Biolegend Cat#506532 Clone B27 RRID:AB_2801092 (BioLegend Cat. No. 506532) Ultra-LEAFTM Purified antihuman IL-6 Antibody Biolegend Biolegend Cat# 501126 Clone MQ2-1385 RRID: AB_2810626 (BioLegend Cat. No. 501126) IL-27 Goat anti-Human, Polyclonal, R&D SystemsTM RnD systems RnD systems Cat# Af2526 Goat anti-human polyclonal antibody (Continued) (Continued). ..



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    Figure 1. The expression of <t>sIL-27Rα</t> level in GVHD patients. (A) Serum sIL-27Rα levels were significantly increased on the day of neutrophil engraftment compared with pre-conditioning (P < 0.0001). (B) The serum sIL-27Rα levels in patients with grade II–IV aGVHD were significantly lower than those of 0-I aGVHD patients on the day of neutrophil engraftment (P < 0.001). (C) On the day of neutrophil engraftment, serum sIL-27Rα levels in patients with skin aGVHD (P < 0.01) and liver aGVHD (P < 0.05) were significantly lower than those of 0-I aGVHD patients. (D) There were no similar significant difference regarding cGVHD. Data shown are mean ± SD.
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    Figure 1. The expression of <t>sIL-27Rα</t> level in GVHD patients. (A) Serum sIL-27Rα levels were significantly increased on the day of neutrophil engraftment compared with pre-conditioning (P < 0.0001). (B) The serum sIL-27Rα levels in patients with grade II–IV aGVHD were significantly lower than those of 0-I aGVHD patients on the day of neutrophil engraftment (P < 0.001). (C) On the day of neutrophil engraftment, serum sIL-27Rα levels in patients with skin aGVHD (P < 0.01) and liver aGVHD (P < 0.05) were significantly lower than those of 0-I aGVHD patients. (D) There were no similar significant difference regarding cGVHD. Data shown are mean ± SD.
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    Journal: Oncoimmunology

    Article Title: T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer

    doi: 10.1080/2162402X.2021.1940675

    Figure Lengend Snippet:

    Article Snippet: IL-27 Goat anti-Human, Polyclonal, R&D SystemsTM , RnD systems , RnD systems Cat# Af2526 Goat anti-human polyclonal antibody.

    Techniques: Purification, Control, Activation Assay, Blocking Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Cell Stimulation, Red Blood Cell Lysis

    Journal: Oncoimmunology

    Article Title: T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer

    doi: 10.1080/2162402X.2021.1940675

    Figure Lengend Snippet:

    Article Snippet: IL-27 Goat anti-Human, Polyclonal, R&D SystemsTM , RnD systems , RnD systems Cat# Af2526 Goat anti-human polyclonal antibody.

    Techniques: Purification, Control, Activation Assay, Blocking Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Cell Stimulation, Red Blood Cell Lysis

    Figure 1. The expression of sIL-27Rα level in GVHD patients. (A) Serum sIL-27Rα levels were significantly increased on the day of neutrophil engraftment compared with pre-conditioning (P < 0.0001). (B) The serum sIL-27Rα levels in patients with grade II–IV aGVHD were significantly lower than those of 0-I aGVHD patients on the day of neutrophil engraftment (P < 0.001). (C) On the day of neutrophil engraftment, serum sIL-27Rα levels in patients with skin aGVHD (P < 0.01) and liver aGVHD (P < 0.05) were significantly lower than those of 0-I aGVHD patients. (D) There were no similar significant difference regarding cGVHD. Data shown are mean ± SD.

    Journal: Scientific reports

    Article Title: Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation.

    doi: 10.1038/s41598-018-28614-4

    Figure Lengend Snippet: Figure 1. The expression of sIL-27Rα level in GVHD patients. (A) Serum sIL-27Rα levels were significantly increased on the day of neutrophil engraftment compared with pre-conditioning (P < 0.0001). (B) The serum sIL-27Rα levels in patients with grade II–IV aGVHD were significantly lower than those of 0-I aGVHD patients on the day of neutrophil engraftment (P < 0.001). (C) On the day of neutrophil engraftment, serum sIL-27Rα levels in patients with skin aGVHD (P < 0.01) and liver aGVHD (P < 0.05) were significantly lower than those of 0-I aGVHD patients. (D) There were no similar significant difference regarding cGVHD. Data shown are mean ± SD.

    Article Snippet: After washing, biotinylated goat anti-human IL-27Rα polyclonal antibody (100 μL/well, Catalogue #BAF1479, R&D Systems) was added as detection antibody for 2 h at room temperature.

    Techniques: Expressing

    Figure 2. The diagnostic value of sIL-27Rα in aGVHD and the association of sIL-27Rα with aGVHD severity, relapse and survival. (A) The area under the ROC curve (AUC) was 0.735 (95% CI 0.618–0.853, P = 0.001) on the day of neutrophil engraftment; when using 59.4 ng/ml as cut-off value from the ROC curve, the sensitivity and specificity were 56% and 81%, respectively. (B) The cumulative incidence of grade II–IV aGVHD was significantly lower in patients with high sIL-27Rα levels by Gray’s test (P = 0.004). (C) Patients with high sIL-27Rα levels showed favourable overall survival compared with patients with low sIL-27Rα levels with Kaplan-Meier survival analysis by log rank test (P < 0.001). (D,E) Patients with high sIL-27Rα levels had lower relapse rate (CIR) and non-relapse mortality (NRM) than did patients with low sIL-27Rα levels by Gray’s test (P = 0.008, respectively).

    Journal: Scientific reports

    Article Title: Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation.

    doi: 10.1038/s41598-018-28614-4

    Figure Lengend Snippet: Figure 2. The diagnostic value of sIL-27Rα in aGVHD and the association of sIL-27Rα with aGVHD severity, relapse and survival. (A) The area under the ROC curve (AUC) was 0.735 (95% CI 0.618–0.853, P = 0.001) on the day of neutrophil engraftment; when using 59.4 ng/ml as cut-off value from the ROC curve, the sensitivity and specificity were 56% and 81%, respectively. (B) The cumulative incidence of grade II–IV aGVHD was significantly lower in patients with high sIL-27Rα levels by Gray’s test (P = 0.004). (C) Patients with high sIL-27Rα levels showed favourable overall survival compared with patients with low sIL-27Rα levels with Kaplan-Meier survival analysis by log rank test (P < 0.001). (D,E) Patients with high sIL-27Rα levels had lower relapse rate (CIR) and non-relapse mortality (NRM) than did patients with low sIL-27Rα levels by Gray’s test (P = 0.008, respectively).

    Article Snippet: After washing, biotinylated goat anti-human IL-27Rα polyclonal antibody (100 μL/well, Catalogue #BAF1479, R&D Systems) was added as detection antibody for 2 h at room temperature.

    Techniques: Diagnostic Assay

    Figure 3. sIL-27Rα as an independent prognostic aGVHD biomarker validated in an independent cohort of 80 patients. (A) The area under the ROC curve (AUC) was 0.790 (95% CI 0.688–0.892, P < 0.001) on the day of neutrophil engraftment, using 59.4 ng/ml as the cut-off value. (B) The cumulative incidence of grade II–IV aGVHD was significantly lower in patients with high sIL-27Rα levels by Gray’s test (P < 0.001). (C) Patients with high sIL-27Rα levels showed favourable overall survival compared with patients with low sIL-27Rα levels on Kaplan-Meier survival analysis by log rank test (P = 0.012). (D,E) Patients with high sIL-27Rα levels had similar relapse rates (CIR) and lower non-relapse mortality (NRM) than did patients with low sIL-27Rα levels by Gray’s test (P = 0.0931, P = 0.005, respectively).

    Journal: Scientific reports

    Article Title: Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation.

    doi: 10.1038/s41598-018-28614-4

    Figure Lengend Snippet: Figure 3. sIL-27Rα as an independent prognostic aGVHD biomarker validated in an independent cohort of 80 patients. (A) The area under the ROC curve (AUC) was 0.790 (95% CI 0.688–0.892, P < 0.001) on the day of neutrophil engraftment, using 59.4 ng/ml as the cut-off value. (B) The cumulative incidence of grade II–IV aGVHD was significantly lower in patients with high sIL-27Rα levels by Gray’s test (P < 0.001). (C) Patients with high sIL-27Rα levels showed favourable overall survival compared with patients with low sIL-27Rα levels on Kaplan-Meier survival analysis by log rank test (P = 0.012). (D,E) Patients with high sIL-27Rα levels had similar relapse rates (CIR) and lower non-relapse mortality (NRM) than did patients with low sIL-27Rα levels by Gray’s test (P = 0.0931, P = 0.005, respectively).

    Article Snippet: After washing, biotinylated goat anti-human IL-27Rα polyclonal antibody (100 μL/well, Catalogue #BAF1479, R&D Systems) was added as detection antibody for 2 h at room temperature.

    Techniques: Biomarker Discovery

    Figure 4. Association of sIL-27Rα with serum cytokine levels in aGVHD. The associations between sIL-27Rα levels with serum IL-27, IL-10, HGF, TNFR1, elafin, REG3α and ST2 levels were analysed by using Spearman’s rank correlation coefficient.

    Journal: Scientific reports

    Article Title: Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation.

    doi: 10.1038/s41598-018-28614-4

    Figure Lengend Snippet: Figure 4. Association of sIL-27Rα with serum cytokine levels in aGVHD. The associations between sIL-27Rα levels with serum IL-27, IL-10, HGF, TNFR1, elafin, REG3α and ST2 levels were analysed by using Spearman’s rank correlation coefficient.

    Article Snippet: After washing, biotinylated goat anti-human IL-27Rα polyclonal antibody (100 μL/well, Catalogue #BAF1479, R&D Systems) was added as detection antibody for 2 h at room temperature.

    Techniques: